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彈性蛋白檢測試劑盒  

利用染料結(jié)合的方法來定量檢測釋放到組織培養(yǎng)液或從生物材料中提取的彈性蛋白。
 
應(yīng)用范圍:樣本可來源于細(xì)胞培養(yǎng)基和含細(xì)胞外基質(zhì)成分的組織提取物(如彈性軟骨、主動脈、皮膚等),可檢測可溶性彈性蛋白原和不可溶性彈性蛋白(抽提后以可溶性彈性蛋白多肽的形式檢測)。
 
樣本要求:樣本體積5-100ul,包含5-75ug的彈性蛋白
 
實(shí)驗(yàn)操作步驟

彈性蛋白的分離:用彈性蛋白沉淀劑處理樣本,使彈性蛋白沉淀下來。
彈性蛋白與染料結(jié)合:裝有沉淀的彈性蛋白管中,加染料和反應(yīng)液,反應(yīng)60min以上
彈性蛋白-染料復(fù)合物獲得:去掉未結(jié)合的染料
復(fù)合物染料的釋放:添加染料釋放劑,使彈性蛋白-染料復(fù)合物中的染料釋放出來
釋放出的染料檢測:繪制標(biāo)準(zhǔn)曲線,513nm或405nm處定量檢測

產(chǎn)品訂購

   product code: f2000        110 assays

   product code: f4000        440 assays

參考文獻(xiàn)

1
allen, r. a., wu, w., yao, m., dutta, d., duan, x., bachman, t. n., champion, h. c., stolz, d. b., robertson, a. m.,
kim, k., et al. (2014) nerve regeneration and elastin formation within poly (glycerol sebacate)-based synthetic arterial grafts one-year post-implantation in a rat model. biomaterials 35, 165–173.
2
anilkumar, t. v., vineetha, v. p., revi, d., muhamed, j. and rajan, a. (2014) biomaterial properties of cholecystderived scaffold recovered by a non-detergent/enzymatic method. journal of biomedical materials research part b:
applied biomaterials.
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augustin, c., frei, v., perrier, e., huc, a. and damour, o. an in vitro selection of new cosmetic active
compounds: from screening tests on monolayered fibroblast culture to efficiency study on 3-d dermal
equivalent. ammonio lattato" attivato 1.
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baiguera, s., del gaudio, c., kuevda, e., gonfiotti, a., bianco, a. and macchiarini, p. (2014) dynamic decellularization and cross-linking of rat tracheal matrix. biomaterials 35, 6344–6350.
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baiguera, s., del gaudio, c., lucali, e., kuevda, e., boieri, m., mazzanti, b., bianco, a. and macchiarini, p. (2014) electrospun gelatin scaffolds incorporating rat decellularized brain extracellular matrix for neural tissue engineering. biomaterials 35, 1205–1214.

詳情點(diǎn)擊:北京群曉科苑生物技術(shù)有限公司

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